The tour begins at the Vismarkt, the historic fish market square whose street pattern may trace its roots to Roman city planning. The adjacent science museum, established in 1934 in the academic heart of this student city, offers collections spanning science, nature, and culture.

A short walk leads to the Forum Groningen, a contemporary cultural center where entry is free. Visitors can explore films, exhibitions, events, and the celebrated rooftop terrace with panoramic views across the city.

The route reaches the Martinikerk, Groningen's oldest church with origins around the year 1000, featuring Romanesque tuff stone construction and Gothic expansions made in the city's economic golden age during the 15th century. Adjacent stands the Martinitoren, the tallest and most recognized tower in Groningen, whose current structure replaced two earlier towers lost to lightning strikes and fire.

The Grote Markt anchors the city center, and the neoclassical Town Hall on its edge stands as a national monument. It was the outcome of the Netherlands' first architectural competition in 1774, though delays caused by French occupation meant it was not put into use until 1810.

The Renaissance-style Goudkantoor of 1635 was built as a provincial tax office, its purpose still declared in the Latin text carved into its facade. Nearby, the Pelstergasthuis dates to at least the 13th century and shelters the oldest bell in Groningen, cast in 1459.

The 1903 Synagogue displays an oriental dome and horseshoe arches inspired by Moorish architecture, reflecting the cultural expression of the Jewish community at the time. The Akerk, an eight-hundred-year-old church originally dedicated to Saint Nicholas, closes the route near the river A where it has served city life since around 1200.