The tour begins with a visit to the House of Virgin Mary above Ephesus, a site of deep significance in Christian tradition and an active pilgrimage destination. According to biblical accounts, St. John the Evangelist accompanied Mary to Ephesus following the crucifixion, and she spent her final years in this modest stone dwelling.

A guided walk through Ephesus covers the State Agora, the Odeon, the Memmius Monument, the Temple of Domitian, Curetes Street, the Trajan Fountain, the Baths of Scholastica, the Temple of Hadrian, the Celsus Library, the Gate of Mithridates and Mazeus, the Commercial Agora, and the Grand Theater. The theater, originally built in the third century BC, was expanded by Rome to accommodate 24,000 spectators in the first century AD.

The day concludes at the Temple of Artemis, counted among the Seven Wonders of the Ancient World by the Greek poet Antipater. Dedicated to the Greek fertility goddess and constructed in the third century BC, it was the first monumental temple built entirely from marble. Guests return to their Kusadasi hotel in the afternoon.

Following hotel pickup, the group travels to Priene, an ancient Greek city built on a terraced hillside above the Meander Valley and centered on the revered Temple of Athena. The route continues to Miletus, a major commercial and political hub from 700 BC through 700 AD, where the Roman Theater and the ornate Faustina Baths remain as principal attractions.

The final stop is Didyma, site of the immense Temple of Apollo, the largest Ionic temple in the ancient world and a place of oracle sacred to the Greeks. The famous cracked Medusa head, which once decorated the temple's frieze, still rests on the ground nearby. Guests are then driven back to their Kusadasi hotel to conclude the tour.